Fig. 5

miR-6794-5p-induced IL-10 in THP-1-derived macrophages contributes to tumorigenicity. A mRNA expression levels of cytokines and growth factors in miR-6794-5p overexpressed THP-1-derived macrophages were examined by qRT-PCR. The values were normalized to GAPDH. B Expression of IL-10 was reconfirmed in the conditioned media of miR-6794-5p overexpressed THP-1 derived macrophages using ELISA. C-F After treatment with recombinant IL-10 (5 ng/ml) on U251 and A549, Western blot (C), wound healing (D), matrigel coated invasion (E), and sphere formation assays (F). β-actin was used for normalization in Western blot analysis. The experiment was repeated with triplicates and representative Western blotting images are shown. Scale bar is 200 μm. G The expression of IL-10 was shown in box and whisker plots by comparing normal (n = 5) and GBM patients (n = 168) from TCGA GBM cohort within the Genomic Data Common (GDC). H The expression of IL-10 in the plasma of normal (n = 22) and patients with lung cancer (n = 19) was assayed by ELISA. Data are presented as mean ± SD after triplicate. *P < 0.05; **P < 0.01; ***P < 0.001. Student’s t-test