Fig. 4
From: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization
miR-6794-5p promotes EMT, cell mobility, invasiveness, and stemness maintenance by suppressing SOCS1. A-D U251 and A549 cells were co-transfected with miR-6794-5p or SOCS1 overexpressing vectors. A The expression of EMT and stemness marker proteins in the indicated cells was confirmed by Western blot analysis. β-actin was used for normalization. The experiment was repeated with triplicates and representative Western blotting images are shown. Wound healing (B), matrigel coating invasion (C), and sphere formation assays (D) were performed to confirm cell mobility, invasiveness, and stemness maintenance in the indicated cells. Scale bar is 200 μm. The data are presented as the mean ± S.D. after triplicate. *P < 0.05; **P < 0.01; ***P < 0.001. One-way ANOVA followed by bonferroni comparison test