Fig. 2
From: Tumor-derived miR-6794-5p enhances cancer growth by promoting M2 macrophage polarization
miR-6794-5p promotes tumorigenicity. A-D U251 and A549 cells were transfected with either negative control (NC) or miR-6794-5p mimic. A Expression of mesenchymal and stemness marker was confirmed with Western blot analysis in indicated cells. β-actin was used for normalization. The experiment was repeated with triplicates and representative Western blotting images are shown. Wound healing (B), matrigel coated invasion (C), and sphere forming (D) assays were subjected on the indicated cells. Scale bar is 200 μm. E-H LLC1 cells overexpressing negative control (NC) or miR-6794-5p inhibitor (anti-miR-6794-5p) were subcutaneously injected into the right flank of Balb/c nude mice (n = 5; 5 X 105 cell/mice), respectively. After harvesting mice on 14 days, whole tumor images (E), tumor volumes (F), and tumor weights (G) obtained from the negative control (NC) and anti-miR-6794-5p overexpressing groups. H The expression of miR-6794-5p in tumor tissue of negative control (NC) and anti-miR-6794-5p overexpressing groups was analyzed by qRT-PCR. The data are presented as the mean ± S.D. after triplicate. *P < 0.05; **P < 0.01; ***P < 0.001. Student’s t-test