Fig. 4

CXCR2 knockout in GECs ameliorates renal glycocalyx degradation by the NF-κB p65 signaling pathway. A Immunofluorescence was used to detect the expression of heparan sulfate in GECs. B Mean intensity of heparan sulfate (× 200, Scale bar = 50 μm, n = 3). C ELISA was used to measure the level of heparan sulfate in the supernatant of four groups of cells. D Immunofluorescence was used to detect the expression of syndecan-1 in GECs. E Mean intensity of syndecan-1(× 200, Scale bar = 50 μm, n = 3). F ELISA was used to measure the levels of syndecan-1 in the supernatant. G and H The protein levels of p-IKKβ, IKKβ, p-IκBα, IκBα, p-NF-κBp65, and NF-κB p65 were detected by western blotting. β-Actin was used as an internal reference control. The immunofluorescence staining (I) and mean intensity of E-selectin (J) (× 200, Scale bar = 50 μm, n = 3). qPCR assay was used to test the level of E-selectin (K). Representative images were shown; original magnification× 200. Results are expressed as mean ± SEM; ***P < 0.001 vs. control group; ^& P < 0.05, ^&& P < 0.01,^&&& P < 0.001 vs. HG group; ^# P < 0.05, ^## P < 0.01, ^### P < 0.001vs. HG + SiCXCR2 group; HG, high glucose