Fig. 2

Glomerular endothelial CXCR2 knockout restores renal function in DKD mice. A Schematic of the experiment. Kidney-related indicators included (B) the urine albumin-to-creatinine ratio (UACR), (C) Kidney weight/body weight (KW/BW), (D) blood urea nitrogen (BUN), and (E) serum creatinine (Scr) were measured (n = 8 per group). Quantification of cross-sectional area of glomerular with hematoxylin and eosin (HE). F Periodic acid-Schiff (PAS)-stain was used for quantifing mesangial matrix fraction (G). H Representative images of HE staining in kidneys. I Representative images of PAS-stained kidneys (× 400; Scale bar = 50 μm, n = 3 mice,five randomly glomeruli were selected per mouse). Results are expressed as mean ± SEM; *P < 0.05, **P < 0.01, and ***P < 0.001 vs. CXCR2 ^L/L group; ^# P < 0.05,^## P < 0.01 and ^### P < 0.001 vs. DKD-CXCR2 ^L/L group, ^ns P > 0.05)