Fig. 1

CXCR2 level is increased in patients with DKD and correlated with glycocalyx damage. Immunohistochemical staining (A) and scores (B) for the expression of CXCR2 are shown in glomeruli and enlarged glomerular image of the box (Scale bar = 50 μm, n = 3 for each group).(C) The mRNA level of CXCR2 in DKD and healthy controls was obtained from www.v5.nephroseq.org of ERCB Database. D A receiver operating characteristic (ROC) curve was used to assess the diagnostic value of CXCR2 for DKD patients based on the data of mRNA level of ERCB Database. Immunohistochemical analysis (E) and quantification (F) of E-selection expression in glomeruli and enlarged glomerular image of the box,positive nuclear staining for E-selection was localized in endocapillary area of enlarged glomerular (Scale bar = 50 μm, n = 3 for each group). Immunofluorescence colocalization (G) and analysis (H) of CXCR2 and CD31 in the glomeruli of human (Scale bar = 10 μm, n = 3, glomeruli were outlined with dotted lines). Immunohistochemical staining (I) and scores (J) for syndecan-1 in glomeruli and enlarged glomerular image of the box (Scale bar = 50 μm, n = 3 for each group). K Line regression was used to assess the correlation between CXCR2 expression and syndecan-1 expression of glomeruli based on the data of IOD in DKD patients and controls (p < 0.001,r² = 0.92). The images for immuohistochemistry:× 630, the images for immunofluorescence:× 400. Error bars indicate SEM, and data represent mean ± SEM. ^**P < 0.01, ^***P < 0.001 vs. control group; IOD,integrated optical density; r², the correlation coefficient; DKD, diabetic kidney disease; UACR, urine albumin creatine ratio; ROC, receiver operating characteristic; AUC, area under the curve