Skip to main content
Fig. 9 | Cell Communication and Signaling

Fig. 9

From: Investigating the therapeutic effects and mechanisms of Carthamus tinctorius L.-derived nanovesicles in atherosclerosis treatment

Fig. 9

CDNVs regulate HUVEC inflammation via the miR166a-3p/CXCL12 pathway. A-B Monocyte adhesion assays were performed, and statistical analysis was conducted. Scale bar: 500 μm. C qPCR assays were conducted to measure the mRNA levels of VCAM-1 and ICAM-1 in HUVECs, and the results were quantified and normalized to GAPDH. D Western blot assays were conducted to measure the protein expression levels of VCAM-1 and ICAM-1 in HUVECs. E Quantification of the protein expression levels of VCAM-1 and ICAM-1 in HUVECs, as normalized to GAPDH, is shown. F-G Monocyte adhesion assays were performed, and statistical analysis was conducted. Scale bar: 500 μm. H qPCR assays were conducted to measure the mRNA levels of VCAM-1 and ICAM-1 in HUVECs, and the results were quantified and normalized to GAPDH. I Western blot assays were conducted to measure the protein expression levels of VCAM-1 and ICAM-1 in HUVECs. J Quantification of the protein expression levels of VCAM-1 and ICAM-1 in HUVECs, as normalized to GAPDH, is shown. The results represent three independent experiments (n = 3). Data represent means ± SD. Significance levels are indicated as *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X