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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: The intracellular interplay between galectin-1 and FGF12 in the assembly of ribosome biogenesis complex

Fig. 4

Galectin-1 modulate formation of FGF12/NOLC1/TCOF1 complexes. PLA-based analysis of galectin-1 impact on FGF12/NOLC1 (A), FGF12/TCOF1 (B), NOLC1/TCOF1 (C) interaction. Confocal images of in situ PLA using anti-FGF12, anti-NOLC1 and anti-TCOF1 antibodies in U2OS-FGF12-GFP.myc cells upon galectin-1 knock-down. Cell nuclei were labeled with NucBlue Live. The dashed line indicates the cell area. The scale bar represents 50 μm. Data shown in the graphs are normalized number of PLA spots in the cell. Single dot represents number of PLA spots recorded in individual cell. Boxes indicate mean ± SEM and lines represents mean from three independent experiments. Statistical analyses were performed with Mann–Whitney U test (*p < 0.05; **p < 0.005 and ***p < 0.001)

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Cell Communication and Signaling

ISSN: 1478-811X