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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: The intracellular interplay between galectin-1 and FGF12 in the assembly of ribosome biogenesis complex

Fig. 1

Galectins interacts with FGF12. A Co-localization of FGF12-GFP.myc with intracellular galectins. To analyze the co-localization of FGF12-GFP.myc with galectin-1,-3,-7 and 8, U2OS-FGF12-GFP.myc cells were fixed, permeabilized and stained with appropriate primary antibodies and Alexa Fluor 594-conjugated anti-rabbit secondary antibody. Cell nuclei were labeled with NucBlue and cells were analyzed by fluorescence microscopy. The scale bar represents 20 μm. B In situ proximity ligation assay (PLA) using mouse anti-myc antibody detecting FGF12-GFP.myc and rabbit antibodies directed against galectin-1,-3,-7, or 8 in U2OS-FGF12-GFP.myc cells. Cell nuclei were labeled with NucBlue and cells were analyzed by fluorescence microscopy. The dashed line indicates the cell area. The scale bar represents 20 μm. Data shown in the graphs are mean normalized PLA signal intensities ± SEM from three independent experiments. Statistical analyses were performed with Kruskal–Wallis H test (*p < 0.05; **p < 0.005 and ***p < 0.001)

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Cell Communication and Signaling

ISSN: 1478-811X