Fig. 5

ACSS2 deletion decreases inflammasome activation and GSDMD-mediated cell pyroptosis in renal tubular cells of LPS-induced mice. A Representative transmission electron microscopy (TEM) images of kidney tissues (Scale bars, 1 μm or 0.5 μm). B Representative immunofluorescent staining of cleaved GSDMD and IL-1β in the kidneys (green, LTL; red, IL-1β or cleaved GSDMD; blue, DAPI; Scale bars, 200 μm). C Western blotting analyses of the protein expression levels of NLRP3, IL-1β, and the cleavage of GSDMD in the kidney tissues of the noted mice (n = 3). Data were presented as mean ± SD. ^*P < 0.05, ^***P < 0.005 vs. Ctrl; ^#P < 0.05, ^##P < 0.01 vs. LPS