Fig. 4

ACSS2 ablation protects kidney function and ameliorates renal tubular injury in LPS-induced mice. A Schematic diagram of the strategy leading to the LPS-induced sepsis mouse model. B Western blotting analyses of the protein expression levels of ACSS2 in renal cortex of mice (n = 3). C Representative immunofluorescent images of ACSS2 and Lotus Tetragonolobus Lectin (LTL) in the kidney tissues from mice (Scale bars, 200 μm). D and E Representative images of (D) H&E staining and (E) immunofluorescent staining of KIM-1 in the kidneys of mice (Scale bars, 100 μm). F The serum levels of BUN and serum creatinine were measured (n = 5-6). G The expressions of F4/80 (a marker for macrophages) in the kidneys were analyzed (Scale bars, 100 μm). Data were presented as mean ± SD. ^*P < 0.05, ^****P < 0.0001 vs. Ctrl; ^#P < 0.05, ^##P < 0.01, ^###P < 0.005 vs. LPS