Fig. 1

The NLRP3 inflammasome and pyroptosis are activated in an LPS-induced mouse sepsis model. A Serum creatinine levels in vehicle-treated mice and LPS-induced sepsis mice were measured (n = 5-6). B H&E staining of kidney tissues from the AKI model mice and control mice (Scale bars, 50 μm). C F4/80 staining of kidney tissues of the noted mice (Scale bars, 200 μm). D Expression of pro-inflammatory factors at the mRNA level in the kidneys of vehicle- or LPS-treated mice were determined using RT-qPCR (n = 5). E The protein expression of NLRP3, cleaved GSDMD, and caspase-1 in the kidney were detected by immunofluorescent staining (green, caspase-1; red, NLRP3 or cleaved GSDMD; blue, DAPI) and Western blotting (n = 3). Data were presented as mean ± SD. ^*P < 0.05, ^**P < 0.01 vs. Ctrl