Fig. 8

Progression of prostatitis in sleep-deprived mice after sleep recovery. A Schematic illustration of sleep recovery for different time (1 to 3 weeks) in SD mice. B Analysis of MT level in serum in SD mice after 3 weeks of sleep-recovery (R1, R2, and R3). C Analysis of DHT level in prostate tissue after 3 weeks of sleep-recovery. D The prostate sizes (prostate index) analysis. E-G ELISA analysis of cytokines in prostate, including IL-6, TNF-α, and PGE2. H-I The concentration of IFN-β in serum and prostate tissue. J HE staining showing the changes of prostate in SD mice after 1 week of sleep-recovery. Scale bar, 100 μm. K The corresponding inflammation score analysis of prostate in HE staining. L Evaluation of tactile allodynia by Von Frey filaments in mice. M-N Western blot and corresponding analysis of the essential proteins of cGAS-STING pathway in prostate of mice after 1 week of sleep-recovery. O Schematic illustration of sleep re-deprivation for different time (1, 3 and 7 days) in mice. P HE staining revealing the changes of prostate in sleep-recovery mice after sleep re-deprivation (R-SD) for different periods (1, 3, and 7 days). Scale bar, 100 μm. Data were presented as means ± SEM (n ≥ 5). Statistical significance was calculated using the one-way ANOVA (B-I, K, L, and N). ns, no significance, *P < 0.05, **P < 0.01, and ***P < 0.001