Fig. 7

Amelioration of prostatitis by inhibiting cGAS-STING pathway in sleep-deprived mice. A Schematic illustration of supplement MT (4 mg/Kg) during the duration of SD in mice for 3 weeks. B HE staining showing the changes of prostate in mice with supplement MT after SD for 3 weeks. Scale bar, 100 μm. C Changes of tactile allodynia by Von Frey filaments. D-E The indexes of oxidative stress in prostate of mice with supplement MT after SD for 3 weeks, including MDA and SOD. F-H ELISA analysis of cytokines in prostate, including IL-6, TNF-α, and PGE2. I-K Immunohistochemical staining and corresponding AOD of p-TBK1 and p-IRF3. Scale bar, 100 μm. L-M The concentration of IFN-β in serum and prostate tissue. N Schematic illustration of the down-regulated cGAS expression by the injection of AAV-cGAS-shRNA into the prostate during the duration of SD in mice for 3 weeks. O Evaluation of cGAS expression in prostate of mice after 3 weeks of AAV-cGAS-shRNA injection. P HE staining showing the changes of prostate in mice after 3 weeks of AAV-cGAS-shRNA injection. Scale bar, 100 μm. Q-S ELISA analysis of cytokines in prostate, including IL-6, TNF-α, and PGE2. T-U The concentration of IFN-β in serum and prostate tissue.Data were presented as means ± SEM (n ≥ 5). Statistical significance was calculated using the one-way ANOVA (C, D-H, J-M, and Q-U). **P < 0.01, and ***P < 0.001