Fig. 5

Activation of cGAS-STING pathway by ROS-mediated mt-DNA release in sleep-deprived mice. A-B Fluorescence images and corresponding mean fluorescence intensity of intracellular ROS in prostate. Scale bar, 10 μm. C-D The indexes of oxidative stress in prostate, including MDA and SOD. E-F Fluorescence microscope images and quantitative analysis of the mitochondrial membrane potential via the changes of aggregates (PE) and JC-1 monomers (FITC). Scale bar, 10 μm. G Morphology of mitochondria of prostate stromal cells. H Fluorescence images of mitochondrial DNA (mt-DNA) in cytoplasm of prostate stromal cells. Scale bar, 50 μm. I-J Western blot and corresponding analysis of the essential proteins of cGAS-STING pathway in prostate. K-L Immunofluorescence images and quantitative analysis of the p-IRF3 expression in prostate stromal cells. Scale bar, 50 μm. Data were presented as means ± SEM (n = 5). Statistical significance was calculated using the Student’s t test (B-D, F, J, and L). **P < 0.01, and ***P < 0.001