Fig. 7

Inhibition of MYC–MAX dimerization destabilizes the cytoskeleton and reduces contractility similar to JQ1. RBMC were plated in 4 well chamber slides and incubated with DMSO, JQ1, MYC-MAX dimerization inhibitors 10,048-F4 (F4), or 10,074-G5 (G5) for 16 h. Cells were stained for αSMA, Phalloidin or DAPI. Forty images were taken and stitched together with ImageJ to make a 5 × 8 field of view. B Phalloidin and αSMA intensity were quantified for each cell using ImageJ and plotted for each condition. C Cells were plated on collagen gels and stimulated for 16 h with DMSO, JQ1, F4 or G5 in serum free media and spontaneous contraction of gels was captured for 1.5 h prior to adding FBS to a final concentration of 5% to elicit contraction. D Contraction was quantified as a percentage in the change in area of the gel compared to baseline. E RNA was harvested from cells stimulated with DMSO, JQ1, F4 and G5 for 16 h and predicted JQ1 sensitive cytoskeleton associated genes was measured with qPCR