Fig. 4
From: Investigation of the impact of bromodomain inhibition on cytoskeleton stability and contraction
Validation of JQ1 sensitive cytoskeletal effectors via qPCR and immunoblots. A subset of cytoskeleton associated targets predicted to be sensitive to JQ1 were validated using qPCR and immunoblotting techniques. Primers for JQ1 sensitive cytoskeleton associated targets were generated using NCBI primer design tool. A time course of vehicle (V), PDGF (P), or TGFB (T) stimulated RBMC with concurrent stimulation of DMSO or JQ1 was performed. A The average Log2FC of three replicates was plotted in a heat map. B The same targets were assessed by immunoblot at 16 h and C quantified. Data are representative of 3 independent trials