Fig. 2

Pattern and enrichment analyses support JQ1 attenuation of genes associated with cytoskeletal changes. Four publicly available datasets in which mechanically active cells were stimulated with a physiologically relevant pro-fibrotic condition in the absence or presence of JQ1 were identified and reanalyzed. Differential gene expression analysis was performed using DESeq2 after generating count matrices from the fastq files using Biogrids software tools including: SRAtoolkit, STAR, Subread. A, C, E Pattern analysis of DEGs from each of the four datasets identified 2 groups of genes that were perturbed by PDGF (GSE11714), Growth medium (GSE138323), or TGFB (GSE127229) and attenuated by JQ1. B, D, F JQ1 sensitive genes were subjected to GO cellular compartment terms and clustered based on common genes between terms. Highlighted in yellow are terms related to chromatin remodeling. Highlighted in green are terms related to cytoskeleton regulation