Fig. 3

CD146⁺CAFs up-regulate IL-10 to facilitate VM. A PCR analysis of angiogensis related cytokines; B IL-10 protein expression in NF, CD146^-CAFs and CD146⁺CAFs by western blot; C IL-10 expression in NF, CD146^-CAFs and CD146⁺CAFs supernatant by ELISA; D The mRNA expression of IL-10 in cancers by Tumor Immune Estimation Resource (TIMER; https://cistrome.shinyapps.io/timer); E The protein expression of VE-Cadherin, E-Cadherin and Vimentin in Ishikawa and KLE after treated with concentration gradient IL-10; F Cord formation assays of Ishikawa and KLE after treated with IL-10 and IL-10 antibody; G The statistical results of cord formation assays