Fig. 3From: Strategies for labelling of exogenous and endogenous extracellular vesicles and their application for in vitro and in vivo functional studiesStrategies for the labelling of endogenously produced EVs for the study of their functional transfer. A Cre-LoxP method. B CRISPR-Cas9 method. C Principle of Cre-LoxP system: Cre-mediated recombination activates a fluorescence switch from DsRed to eGFP in reporter+ cells after receiving Cre+-EVs produced by Cre+ cells. D Mechanism of CRISPR-Cas9: Reporter+ cells expressing Cas9 switch from mCherry to eGFP fluorescence after functional uptake of EVs. EVs produced by genetically manipulated donor cells carry a specific targeting single guide RNA (sgRNA) that navigates Cas9 with nuclease activity to generate cleavage of the target sequenceBack to article page