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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: Strategies for labelling of exogenous and endogenous extracellular vesicles and their application for in vitro and in vivo functional studies

Fig. 3

Strategies for the labelling of endogenously produced EVs for the study of their functional transfer. A Cre-LoxP method. B CRISPR-Cas9 method. C Principle of Cre-LoxP system: Cre-mediated recombination activates a fluorescence switch from DsRed to eGFP in reporter+ cells after receiving Cre+-EVs produced by Cre+ cells. D Mechanism of CRISPR-Cas9: Reporter+ cells expressing Cas9 switch from mCherry to eGFP fluorescence after functional uptake of EVs. EVs produced by genetically manipulated donor cells carry a specific targeting single guide RNA (sgRNA) that navigates Cas9 with nuclease activity to generate cleavage of the target sequence

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Cell Communication and Signaling

ISSN: 1478-811X