Fig. 5

O-GlcNAcylation of STING activates antiviral signaling. A and B Downstream targets in STING signaling were detected with immunoblotting in shSTING cells reconstituted with either STING-WT or STING-T229A followed by challenges with HSV-1 (MOI = 1) or poly(dA:dT) (2 μg/mL) for 16 h. C and D Downstream targets in STING signaling in STING-WT- or STING-T229A-reconstituted shSTING cells challenged with poly(dA:dT) in the presence or absence of TMG (10 μM, O-GlcNAc activator) for 12 h. Data are representatives from 3 independent experiments