Fig. 9

MDA-MB-468 cells acquire resistance to MAPK inhibition. A MDA-MB-468 cells were seeded and cultivated in phenol red-free RPMI 1640 for 24 h. On the following day, cells were treated with U0126 (10 µM) for the indicated times. Subsequently, cells were lysed and proteins were separated by SDS-PAGE. After Western blotting, membranes were stained with specific antibodies against (p)Y Gab1, Gab1, (p)T/Y ERK1/2, ERK1/2, (p)S Akt, Akt, (p)Y STAT3, STAT3 and tubulin. A representative result of n = 3 independent experiments is shown. The results from (A) were quantified. The diagrams show the ratios of B (p)Y Gab1 to Gab1, C (p)T/Y ERK1/2 to ERK1/2, D (p)S Akt to Akt, and E (p)Y STAT3 to STAT3. Gab1, ERK1/2, Akt and STAT3 phosphorylation in untreated cells was normalised to 100% in each independent repetition of the experiment. Data are given as mean of three independent experiments ± SD. One-way ANOVA: n.s. = non-significant, * = p < 0.05, ** = p < 0.01, *** = p < 0.001