Fig. 6

PI3K signalling is crucial for proliferation and migration of MDA-MB-468 cells. MDA-MB-468 cells were seeded on 6-well plates and cultivated in phenol red-free RPMI 1640. On the following day, cells were treated with DMSO, U0126 (10 µM) and/or LY294002 (40 µM) A After 72 h the total cell number was determined. Proliferation is depicted as % increase of proliferation compared to d 0. B After 72 h, the percentage of viable cells was determined. C MDA-MB-468 cells were seeded on 24-well plates and cultivated in phenol red-free RPMI 1640. The next day, the cells were subjected to a wound healing assay upon treatment with DMSO, U0126 (10 µM) and/or LY294002 (40 µM) for 72 h. Wound closure is given in % of initial wound area. Data are given as mean of n = 3 independent experiments ± SD. One-way ANOVA: n.s. = non-significant, * = p < 0.05, ** = p < 0.01, *** = p < 0.001