Fig. 3

EGFR activity promotes migration of MDA-MB-468 cells. MDA-MB-468 cells were seeded on 6-well plates and cultivated in phenol red-free RPMI 1640. On the following day, cells were treated with DMSO or Gefitinib (3 µM). A After the time indicated, the total cell number was determined and the cell number at day 0 was normalised to 100%. B The percentage of viable cells was determined at the indicated time points. C MDA-MB-468 cells were seeded on 24-well plates and cultivated in phenol red-free RPMI 1640. After 24 h, the cells were subjected to a wound healing assay upon DMSO or Gefitinib (3 µM) treatment for 72 h. Representative results of n = 3 independent experiments are shown. D Wound closure is given in % of initial wound area. Data are given as mean of n = 3 independent experiments ± SD. One-way ANOVA: n.s. = non-significant, * = p < 0.05, ** = p < 0.01, *** = p < 0.001