Fig. 11
From: Dysregulated Gab1 signalling in triple negative breast cancer
Gab1 is localised in the cytoplasm in PI3K and MAPK inhibition resistant MDA-MB-468 cells. MDA-MB-468 cells were seeded on poly-L-lysine-coated glass cover slips and cultivated in phenol red-free RPMI 1640. After 24 h, cells were transfected with an expression vector for murine Gab1-GFP. On the following day, cells were treated with DMSO, U0126 (10 µM) or LY294002 (40 µM). After 48 h cells were placed into the incubation chamber of a laser scanning microscope. Imaging was performed after 30 min equilibration. Representative results of n = 3 independent experiments are shown