Fig. 10
From: Dysregulated Gab1 signalling in triple negative breast cancer
Inhibition of PI3K and MAPK affects mRNA expression in a gene-specific manner. MDA-MB-468 cells were seeded and cultivated in phenol red-free RPMI 1640 for 24 h. On the following day, cells were treated with DMSO, U0126 (10 µM) or LY294002 (40 µM) for the indicated times. mRNA was isolated and transcribed into cDNA. EGR1 mRNA (A, B) and cyclin D1 mRNA (C, D) were quantified by qRT-PCR. Expression of mRNA in DMSO-treated cells was set to 100%. Data are given as mean of n = 3–4 independent experiments ± SD. Student’s t-test: n.s. = non-significant, * = p < 0.05, ** = p < 0.01, *** = p < 0.001