Fig. 7

TMEM160 is associated with PD-L1 expression in human CRC and predicts poor patient prognosis. A Western blotting assays were employed to detect the protein levels of TMEM160 in eight pairs of CRC tissue samples. B IHC analysis was conducted to detect the protein levels of TMEM160 in tumor tissues (n = 125) and adjacent normal tissues (n = 125) obtained from patients clinically diagnosed with CRC. C The patients with CRC were categorized into groups based on the IHC results of TMEM160 protein levels. Kaplan–Meier analysis was then conducted to assess the survival outcomes in each group. D IHC analysis was performed on serial sections of both tumor tissues and paired normal tissues. The purpose of the analysis was to evaluate the expression levels of TMEM160, PD-L1, and CD8A proteins. To assess the correlation between TMEM160 expression and the expression of PD-L1 and CD8A, a chi-square test was employed. E The correlation between TMEM160 protein expression and the protein expression of PD-L1 and CD8A was analyzed using IHC assays performed on CRC tissues that were obtained from clinical patients. F Kaplan–Meier survival analysis was performed on a cohort of 125 patients, who were classified into four groups based on the protein levels of TMEM160 and PD-L1