Fig. 5
Deletion of TMEM160 impairs the role of PD-L1 in T cell-mediated tumor cytotoxicity and reduces radiotherapy resistance in CRC cells. A The association of TMEM160 with immunity was predicted using the TIMER database. B Schematic representation of TMEM160 correlation with immune infiltration. C FACS analysis of Jurkat cell-mediated killing of tumor cells using Annexin V and propidium iodide (PI) double staining. D The quantitative measurement of apoptotic cells in the Q2 and Q3 quadrants of each group, with more than 104 cells counted in each group. E CCK8 analysis was performed to detect tumor cell viability after incubation with activated Jurkat cells. F, G To investigate the protein levels of TMEM160 and PD-L1 after radiotherapy (RT), measurements were performed in two experimental groups: siTMEM160#1 and TMEM160 overexpression. Both groups were subjected to 8 Gy of radiation. H, I The colony formation assays were visually represented through representative photographs, depicting the surviving proportions of DLD1 and RKO cells following irradiation with 0, 2, 4, and 8 Gy. J, K The proliferation of DLD1 and RKO cells was assessed using CCK8 assays. The independent biological experiments were repeated three times. Statistical significance is denoted *p < 0.05, **p < 0.01 and ***p < 0.001