Fig. 4

TMEM160 promotes malignant biological behavior in CRC cells. After the knockdown of TMEM160 in HCT116 and DLD1 cells using siTMEM160#1 and siTMEM160#2. A, C The CCK-8 assay demonstrated that cell proliferation was inhibited when TMEM160 was reduced. B, D The results of the transwell assay indicated a significant reduction in both cell migration and invasion. E The colony formation ability of CRC cells was significantly impaired when TMEM160 expression was downregulated. F-J SW480 and RKO cells that were stably transfected with Myc-TMEM160 plasmids exhibited significant enhancements in CRC cell proliferation (F, H), migration, invasion (G, I), and colony formation abilities (J). Independent biological experiments were repeated three times each, helping to ensure the reliability and reproducibility of the results. Statistical significance is denoted by *p < 0.05 and **p < 0.01. Scale bar, 100 μm