Fig. 1

TMEM160 interacts with PD-L1. A The BioGRID database was used to search for the interacting proteins of CD274. B HCT116 cells were transfected using Flag-PD-L1 and Myc-TMEM160 for immunostaining with Flag antibodies (green), Myc antibodies (red), and DAPI antibodies (blue). Scale bar, 20 µm. C A prediction model for TMEM160 was obtained from the UniProt database (left), while a prediction model for PD-L1 was obtained from the Protein Data Bank (PDB) database (right). D The predicted interface of one of the complexes formed by TMEM160 and PD-L1. E, F Results of Co-IP of exogenous PD-L1 and TMEM160 in HEK-293 T and SW480 cells. G, H Endogenous Co-IP assays were performed in DLD1 and HCT116 cells to assess the interaction of PD-L1 with TMEM160. I The GST pull-down assay was conducted to confirm the direct binding between the His-PD-L1 and the GST-TMEM160 fusion protein