Fig. 6
From: Antidepressant fluoxetine alleviates colitis by reshaping intestinal microenvironment
Clustering and pseudoprime analysis of monocyte-macrophages from mouse intestinal mucosa. AVisualization(UMAP plots) of monocyte-macrophage single-cell transcriptomes in mouse intestinal mucosa. B A UMAP plot showing 10 clusters and population annotations of monocyte-macrophages. C A UMAP plot showing the expression of canonical genes among macrophage markers. D Heatmap showing the top 20 markers (or all markers if less than 20) (by average log[fold change]) for each of the 10 clusters. E A UMAP plot showing 6 monocyte-macrophage clusters. F Cell proportion distributions of 9 monocyte-macrophage types in the intestinal mucosa of NC/DSS/DSS + Fluoxetine groups. G Pseudptime analysis of monocyte-macrophages showing development trajectories from monocytes to different types of M1 macrophages. H Gene expression dynamics for two trajectories in (G) along development pseudotime. I,J Predicted interaction of all the clusters between different types of macrophages. K KEGG enrichment analysis based on marker genes in different clusters