Fig. 3

Fluoxetine significantly alleviates colon inflammation. A Establishment of an ulcerative colitis animal model. The fluoxetine group was intraperitoneally injected with fluoxetine solution at 10 mg/kg for 100uL, while the DSS group was intraperitoneally injected with an equal volume of PBS. Fluoxetine was dissolved in PBS. After 14 days, all mice in the DSS group and the fluoxetine group received 3% (wt/vol) DSS dissolved in drinking water for 7 days. B Body weight changes. C Representative images of the overall appearance and length of the colon in different groups. D DAI scores. E Representative microscopic images of H&E staining and histology scores. F Representative images of MUC2 immunohistochemical staining of colonic sections. The scar bar represents 50 μm. G RT-qPCR of the intesinal barrier and inflammation-associated genes. H Western-blot results of intestinal barrier-associated genes. I Representative fluorescent images of ZO-1 staining of colonic sections. Scale bars represent 50 μm