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Fig. 8 | Cell Communication and Signaling

Fig. 8

From: The in vitro and in vivo depigmentation activity of coenzyme Q0, a major quinone derivative from Antrodia camphorata, through autophagy induction in human melanocytes and keratinocytes

Fig. 8

CoQ0 inhibited melanogenesis by inducing autophagy in α‐MSH-stimulated B16F10 cells. A-C Cells were treated with CoQ0 (0 or 5 μM) in the absence or presence of 3-MA (1 mM, 1 h) followed by stimulation with α‐MSH (1 μM) for 24 h (A, B) or 72 h (C). A An immunoblotting assay was applied to measure the tyrosinase expression. B Tyrosinase enzyme activity was measured as described in Materials and Methods. C Intracellular melanin levels were quantified. The results are the mean ± SD (n=3). *p < 0.05; ***p < 0.001 compared with untreated cells. #p < 0.05; ###p < 0.001 compared with CoQ0-treated cells. D Cells were treated with or without 3-MA (1 mM, 1 h) followed by treatment with CoQ0 (0 or 5 μM) and stimulation with α‐MSH (1 μM, 24 h). Melanosome-engulfing autophagosomes within cells were observed under TEM. The blue and red arrows indicate melanosome-engulfing autophagosomes, and autolysosomes formation, respectively.

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Cell Communication and Signaling

ISSN: 1478-811X