Skip to main content
Fig. 5 | Cell Communication and Signaling

Fig. 5

From: The in vitro and in vivo depigmentation activity of coenzyme Q0, a major quinone derivative from Antrodia camphorata, through autophagy induction in human melanocytes and keratinocytes

Fig. 5

CoQ0 induced autophagic flux in B16F10 cells. A, B Cells were treated with CoQ0 (0-5 μM, 24 h). After incubation, the cells were subjected to an immunoblotting assay to determine LC3-I/II, ATG4B, and ATG7 protein levels. C, D The GFP-LC3 expression vector was transfected into the cells, followed by treatment with CoQ0 (0-5 μM, 24 h). GFP-LC3 puncta induced by CoQ0 were observed using a confocal microscope. The quantification of cells developing GFP-LC3 puncta is presented in a histogram. The results are the mean ± SD (n=3). *p < 0.05; ***p < 0.001 compared with untreated cells. ###p < 0.001 compared with CoQ0-treated cells

Back to article page

Cell Communication and Signaling

ISSN: 1478-811X