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Fig. 4 | Cell Communication and Signaling

Fig. 4

From: The in vitro and in vivo depigmentation activity of coenzyme Q0, a major quinone derivative from Antrodia camphorata, through autophagy induction in human melanocytes and keratinocytes

Fig. 4

CoQ0 suppressed MITF nuclear translocation in B16F10 cells through the ERK, JNK, and PI3K/AKT signaling pathways. A, B Cells were first treated with inhibitors of ERK (PD98059, 30 μM), JNK (SP600125, 25 μM), p38 (SB203580, 20 μM), PKC (GF109203X, 2.5 μM), or PI3K/AKT (LY294002, 30 μM) for 1 h followed by CoQ0 (5 μM, 4 h). Levels of p-MITF, and nuclear/cytosolic MITF were determined using immunoblotting. C Cells were treated with CoQ0 (5 μM, 0-120 min). An immunoblotting assay was performed to determine the levels of the p-ERK1/2, ERK1/2, p-JNK1/2, JNK1/2, p-PI3K, PI3K, p-AKT, and AKT proteins. D Cells were treated with CoQ0 (0-5 μM) for the indicated time, and immunoblotting was performed to determine the levels of the p-ERK1/2 (90 min), ERK1/2 (90 min), p-JNK1/2 (30 min), JNK1/2 (30 min), p-PI3K (60 min), PI3K (60 min), p-AKT (60 min), and AKT (60 min) proteins. The results are the mean ± SD (n=3). ***p < 0.001 compared with untreated cells. ###p < 0.001 compared with CoQ0-treated cells

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Cell Communication and Signaling

ISSN: 1478-811X