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Fig. 3 | Cell Communication and Signaling

Fig. 3

From: The in vitro and in vivo depigmentation activity of coenzyme Q0, a major quinone derivative from Antrodia camphorata, through autophagy induction in human melanocytes and keratinocytes

Fig. 3

CoQ0 suppressed melanogenesis in α-MSH-stimulated B16F10 cells. A Cells were incubated with CoQ0 (0-7.5 μM) for 24, 48, or 72 h, and an MTT assay was conducted to determine cell viability. B Cells were treated with CoQ0 (0-5 μM) for the indicated durations, and the levels of MC1R (5 min), p-CREB (1.5 h), CREB (1.5 h), p-MITF (4 h), MITF (4 h), and tyrosinase (24 h) were measured using immunoblotting. C Cells were treated with CoQ0 (0-5 μM, 72 h), and melanin formation was determined as described in the Methods section. D, F Cells were first treated with CoQ0 (0-5 μM), followed by stimulation with α-MSH (1 μM) for the indicated time to measure (D) MC1R (5 min), p-CREB (1.5 h), CREB (1.5 h), p-MITF (4 h), and MITF (4 h) levels; (E) nuclear and cytosolic MITF (4 h) levels; and (F) tyrosinase (24 h), TRP-1 (24 h), and TRP-2 (24 h) levels using immunoblotting. G, H Tyrosinase activity was determined after 24 h, and melanin levels were measured after 72 h, as described in the Methods section. Arbutin (ARB, 200 μM) was used as a positive control. The results are the mean ± SD (n=3). *p < 0.05; **p< 0.01; ***p < 0.001 compared with untreated cells. #p < 0.05; ###p < 0.001 compared with CoQ0-treated cells

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Cell Communication and Signaling

ISSN: 1478-811X