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Fig. 13 | Cell Communication and Signaling

Fig. 13

From: The in vitro and in vivo depigmentation activity of coenzyme Q0, a major quinone derivative from Antrodia camphorata, through autophagy induction in human melanocytes and keratinocytes

Fig. 13

CoQ0 triggered antimelanogenesis and melanin degradation in zebrafish embryos. A-E Zebrafish embryos, 9 hpf, were treated with or without 3-MA (1 mM), followed by vehicle (0.1% DMSO) or CoQ0 (0-15 μM) up to 72 hpf. A, B The viability (%) and heart rate (beats/min) of zebrafish were measured using a stereomicroscope. C, D CoQ0 suppressed melanogenesis in zebrafish. PTU (4.4 μM) served as a positive control. E At the end of treatments, proteins were extracted, and an immunoblotting assay was conducted to measure LC3-II and tyrosinase expression. F, G CoQ0 triggered melanin degradation in zebrafish. Zebrafish at 72 hpf were treated with or without 3-MA (1 mM) followed by CoQ0 (0 or 10 μM) for 24 h (72+24 hpf) with or without α-MSH (1 μM) stimulation. The change in endogenous body pigmentation (melanin levels) in zebrafish (lateral views are shown) was measured as described in the methodology section. The results are the mean ± SD (n=3). *p < 0.05; **p < 0.01; ***p < 0.001 compared with control zebrafish embryos. ##p < 0.01; ###p < 0.001 compared with CoQ0-treated zebrafish

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Cell Communication and Signaling

ISSN: 1478-811X