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Fig. 12 | Cell Communication and Signaling

Fig. 12

From: The in vitro and in vivo depigmentation activity of coenzyme Q0, a major quinone derivative from Antrodia camphorata, through autophagy induction in human melanocytes and keratinocytes

Fig. 12

CoQ0 suppressed melanosome associated gp100 expression and triggered melanin degradation by inducing autophagy in melanin-feeding HaCaT cells. Melanin-treated HaCaT cells were pretreated with or without 3-MA (1 mM, 1 h), followed by treatment with CoQ0 (0-5 μM, 24 or 72 h). A The gp100 and LC3B expressions (24 h) were determined using immunoblotting. B, C The gp100 expression (24 h) as assessed by immunofluorescence staining. D The intracellular melanin levels (72 h) were estimated using the procedures described in the methodology section. The results are the mean ± SD (n=3). **p < 0.01; ***p < 0.001 compared with untreated cells. ##p< 0.01; ###p < 0.001 compared with CoQ0-treated cells. E TEM was used to analyze the CoQ0 promoted formation of melanosome-engulfing autophagosomes, and autolysosomes. Melanin-treated HaCaT cells were pretreated with or without 3-MA (1 mM, 1 h) followed by CoQ0 (0 or 5 μM, 24 h). M = mitochondria. The blue, yellow, and red arrows indicate melanin/melanosomes, autophagosomes containing melanin/melanosomes, and autolysosomes, respectively

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Cell Communication and Signaling

ISSN: 1478-811X