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Fig. 10 | Cell Communication and Signaling

Fig. 10

From: The in vitro and in vivo depigmentation activity of coenzyme Q0, a major quinone derivative from Antrodia camphorata, through autophagy induction in human melanocytes and keratinocytes

Fig. 10

CoQ0 modulated autophagy-associated proteins in HaCaT cells. Cells were treated with CoQ0 (0-5 μM, 24 h). A Cell viability was examined by MTT assay. B Cells were subjected to immunoblotting to determine LC3-I/II, ATG4B, ATG5, and ATG7 protein levels. C, D Cells were first treated with or without 3-MA (1 mM, 1 h) followed by treatment with CoQ0 (0-5 μM, 24 h). Immunofluorescence staining was performed to determine LC3B levels. E, F Cells were treated with CoQ0 (0-5 μM, 24 h) and then subjected to immunoblotting to determine Beclin-1 and Bcl-2 protein levels. Data are presented as the Beclin-1/Bcl-2 ratio. The results are the mean ± SD (n=3). **p < 0.01; ***p < 0.001 compared with untreated cells. ###p < 0.001 compared with CoQ0-treated cells

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Cell Communication and Signaling

ISSN: 1478-811X