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Fig. 6 | Cell Communication and Signaling

Fig. 6

From: Phosphorylation of PP2Ac by PKC is a key regulatory step in the PP2A-switch-dependent AKT dephosphorylation that leads to apoptosis

Fig. 6

Using PLA to follow PP2Ac interaction with AKT, PI3K and IGBP1. A αT3-1 cells were cultured on cover slips. The cells were transfected with either HA-PP2Ac or PP2Ac mutants (S24A, S24E) in PCDNA3 in serum starvation medium (0.1% FCS 16 h). The cells were then either stimulated with TPA (250 nM, 30 min) or left untreated (NT) and fixed. Interactions were detected and quantified using the PLA kit, with anti HA for PP2Ac isoforms together with each of the other specific Abs (AKT, p85-PI3K and IGBP1) as described in the Method section. B The bar graphs represent means ± SD of a representative experiment that was reproduced 3 times. Significance of change from non-stimulated control (NT) is calculated. * p < 0.01, ** p < 0.05

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Cell Communication and Signaling

ISSN: 1478-811X