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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Phosphorylation of PP2Ac by PKC is a key regulatory step in the PP2A-switch-dependent AKT dephosphorylation that leads to apoptosis

Fig. 2

Identification of phosphorylation sites on PP2Aa and PP2Ac by MS. PC3 cells were serum-starved (0.1% FCS, 16 h). The cells were stimulated with TPA (250 nM, 30 min), and then endogenous PP2Ac was IPed using monoclonal anti-PP2Ac Ab (BD Transduction Laboratories), and subjected to LC–MS analysis. A HCD spectrum of the doubly charged ion (M/Z-499.72) interpreted as QLS(p)ESQVK phosphorylated S24 of PPP2Ac. B HCD spectrum of the triply charged ion (M/Z-669.013) interpreted as QLSQS(p)LLPAIVELAEDAK phosphorylated S403 of PPP2R1A. b ions are marked in red, y ion in blue, and the precursor in green

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Cell Communication and Signaling

ISSN: 1478-811X