Fig. 5

Double inhibition of PAP and CD73 in male mice, mitigated glycolytic shift and proinflammatory response. A-C Representative Immunoblotting of CD73, TNAP, and PAP and their respective Graphical plots; each blot band in the representative blot is an independent biological sample (n = 3 hearts per group). E ELISA analysis of Extracellular Adenosine level (n = 4 mice/group). F-H Representative Oil Red O (ORO) and Periodic Acid Schiff (PAS) staining of myocardial sections and their respective graphical presentations showing lipid and glycogen deposition percentages (n = 4–6 sections per 4–5 mice per group) Scale bar, 50 μm. I-K Representative Immunoblotting of CD36 and Glut1 and their respective Graphical plots; each blot band in the representative blot is an independent biological sample (n = 3 hearts per group). L-N Representative immunohistochemical staining and graphical presentation of macrophage CD86 and CD206-positive cells assessed from the myocardial sections (n = 4–5 hearts per group) Scale bar, 50 μm. O-Q Inflammatory cytokines; Interleukin TNF-α, IL-10, and transforming growth factor (TGF)-β concentrations assessed by ELISA using myocardia lysates. All ELISA were performed in triplicates (n = 4–5 mice per group). M = male; MH = male + HH; F = female; FH = female + HH; MI = male + APCP; FI = female + APCP; MHI = male + HH + APCP; FHI = female + HH + APCP. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001