Fig. 3

CD73/adenosine axis mediates immunomodulation during HH in a sex-dependent manner. A-B ELISA analysis of Extracellular Adenosine level and CD73 activity (n = 4 mice/group). C Relative mRNA levels of adenosine receptors (A1, A2a, A2b, A3 and n = 4mice/group). D-F Representative immunohistochemical staining and graphical presentation of macrophage CD86 and CD206-positive cells assessed from the myocardial sections (n = 4–6 hearts per group) Scale bar, 50 μm. G-K Graphical presentation of sera cardiac troponin I (cTnI) concentrations and inflammatory cytokines; Interleukin (IL)-1β, IL-18, IL-10, and transforming growth factor (TGF)-β concentrations assessed by ELISA and RT-qPCR using myocardia lysates. All ELISA were performed in triplicates (n = 5–6 mice per group). M = male; MH = male + HH; F = female; FH = female + HH; MI = male + APCP; FI = female + APCP; MHI = male + HH + APCP; FHI = female + HH + APCP. Data are presented as mean ± SEM; ***p < 0.001; **p < 0.01; *p < 0.05