Fig. 2

Migratory phenotypes induced by the P373L and P373R E-cadherin variants. (A) Scheme elucidating single cell and collective motile features extracted from images of time-lapse microscopy. (B) Representative migration patterns of cells expressing wild-type E-cadherin, P373L HDGC and P373R OFC variants at different time points. (C) Overview of cell migratory trajectories throughout 24 h. (D) Travelled distance, (E) mean-square displacement (MSD), and (F) diffusion rates at different time points, were calculated for individual cells. Graphs representing (G) speed of gap closure and (H) covered area over time. (I) Wound gap regularity determined from the distance between the initial position and the leading edge, 6 h upon device removal. Data includes results from four independent experiments. Error bars depict standard error of the mean. # represents a significant difference between P373L HDGC and P373R OFC expressing cells (P < 0.05)