Fig. 1

Motile and morphological features of border cells expressing HDGC and OFC variants. (A) Schematic representation of E-cadherin comprising the signal peptide, precursor, extracellular, transmembrane, and cytoplasmic domains. The location of D254H and P373L variants found in HDGC context, as well as D254N and P373R found in OFC patients is indicated. (B) Illustration of border cell migration from anterior to posterior pole of the Drosophila egg chamber. (C) Migration phenotypes of the different E-cadherin mutants in stage 10 egg chambers. Border cells are marked with GFP (green), phalloidin (red) stains F-actin, and the nuclei are counterstained with DAPI (blue). (D) Quantification of border cell migration index (n = 30 for each phenotype). (E) Images depict the morphology of border cell cluster at 50–70% of travelled distance, and corresponding close up. (F) Border cell cluster and morphological parameters analysed. (G) Average length and (H) sphericity of border cell clusters. Data derives from four independent experiments and * represents P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001