Fig. 9
RNA sequencing revealed significant upregulation of calcium-regulated transcriptional activators and of genes involved in cytokine signaling in 32D-JAK2-V617F/EpoR upon EPO stimulation. (A) Volcano plot represents overall transcriptional change after EPO treatment. Each data point in the scatter plot represents a gene. The log2 fold change of each gene is represented on the x-axis and the log10 of its adjusted p-value is on the y-axis. Genes with an adjusted p-value less than 0.05 and a log2 fold change greater than 1 are indicated by red dots. These represents significantly up-regulated genes. (Note: There were no downregulated genes observed) (B) Bi-clustering heatmap showing the expression profile of the top significantly differentially expressed genes sorted by their adjusted p-value by plotting their log2 transformed expression values in samples (C) Network interaction image showing the tight interaction between the identified upregulated genes. The number of strings between the connected genes indicate the strength of their interaction. The colours of the spheres designate the biological processes in which the genes participate