Fig. 7

BAPTA treatment induces apoptosis in 32D-JAK2-V617F cells. A Western blot analysis of PLCγ-1 after BAPTA treatment in 32D-JAK2-V617F/EPOR cells. Numbers represent values normalized to the respective total protein. B Representative flow cytometric dot plots showing the effect of starvation, EPO stimulation and inhibitory effect BAPTA on early (right lower quadrant) and late (right upper quadrant) apoptosis in 32D-JAK2-V617F/EpoR cells. Data represents mean ± SEM from 3 independent experiments. Statistical analysis by one-way ANOVA by Tukey’s multiple comparison test. * p ≤ 0.05; ** p < 0.01.