Fig. 6

Inhibition of PLCγ-1 triggers apoptosis in 32D-JAK2-V617F cells. Representative flow cytometric dot plots showing the effect of starvation, EPO stimulation and the inhibitory effect of U-73122 or U-73343 on early (right lower quadrant) and late (right upper quadrant) apoptosis in 32D-JAK2-V617F/EpoR cells. Bar data represents mean (sum of early and late apoptosis) ± SEM from 3 independent experiments. Statistical analysis by one-way ANOVA by Tukey’s multiple comparison test. *** p ≤ 0.001