Fig. 5
The inhibition of PLCγ-1 effectively inhibits the proliferation of 32D-JAK2-V617F cells induced by EPO. (A) Cell proliferation assay of 32D-JAK2-WT/EpoR and 32D-JAK2-V617F/EpoR cells after EPO stimulation (1 IU/ml). Data represents mean ± SEM from 3 independent experiments. Statistical analysis by two-way ANOVA by Tukey’s multiple comparison test. ns p > 0.05; **** p ≤ 0.0001. (B) Western blot analysis of PLCγ-1 after using PLC inhibitor (U-73122) and inactive analog (U-73343) of U-73122 in 32D-JAK2-V617F/EPOR cells. Numbers represent values normalized to the respective total protein. (C) Cell proliferation assay of 32D-JAK2-V617F/EpoR cells after concomitant treatment with EPO stimulation (1 IU/ml) and U-73122 or U-73343. Data represents mean ± SEM from 3 independent experiments. Statistical analysis by two-way ANOVA by Tukey’s multiple comparison test. **** p ≤ 0.0001