Fig. 4

Increased baseline Ca²⁺ levels under JAK2-WT and CALR-WT condition. (A) Schematic illustration of the experimental design of the study describing the basal calcium level measurement in 32D-JAK2-WT/EpoR and 32D-JAK2-V617F/EpoR cells under steady state condition and pre-EPO stimulation. (B) Schematic illustration of the experimental design of the study describing the basal calcium level measurement in 32D-CALR-WT/MPL and 32D-CALR-ins5/MPL and 32D-CALR-del52/MPL cells pre-TPO stimulation. (C) & (D) Increased basal Ca²⁺ levels in 32D-JAK2-WT/EpoR cells. Data represents mean ± SEM from 6 independent experiments. Statistical analysis by Mann–Whitney-U-test (for Figure C) and unpaired Student’s t-test (for Figure D). **p < 0.01; **** p ≤ 0.0001 (E) Increased basal Ca.²⁺ levels in 32D-CALR-WT/MPL cells. Data represents mean ± SEM from 5 independent experiments. Statistical analysis by unpaired Student’s t-test. **p < 0.01; **** p ≤ 0.0001